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M9490666.TXT
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1994-09-24
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Document 0666
DOCN M9490666
TI Simultaneous determination of primaquine and carboxyprimaquine in plasma
using high-performance liquid chromatography with electrochemical
detection.
DT 9411
AU Dean RA; Ochieng W; Black J; Queener SF; Bartlett MS; Dumaual NG;
Department of Pathology and Laboratory Medicine, School of; Medicine,
Indiana University, Indianapolis.
SO J Chromatogr B Biomed Appl. 1994 Apr 22;655(1):89-96. Unique Identifier
: AIDSLINE MED/94340105
AB A selective and sensitive high-performance liquid chromatographic method
with electrochemical detection is described for the simultaneous
quantitation of primaquine and carboxyprimaquine, its primary
metabolite, in plasma. After addition of internal standard, plasma was
deproteinized by addition of acetonitrile. Nitrogen-dried supernatants,
resuspended in mobile phase were analyzed on a C8 reversed-phase column.
Limits of detection for primaquine and carboxyprimaquine were 2 and 5
ng/ml with quantitation limits of 5 and 20 ng/ml, respectively. None of
47 tested antimicrobial agents interfered. In contrast to previously
reported methods, the assay sensitivity and specificity are sufficient
to permit quantitation of primaquine in plasma for pharmacokinetics
following low dose (30 mg, base) oral administration of primaquine,
typically used in the treatment of malaria and Pneumocystis carinii
pneumonia.
DE Acquired Immunodeficiency Syndrome/DRUG THERAPY Chromatography, High
Pressure Liquid Drug Combinations Electrochemistry Human Indicators
and Reagents Primaquine/*ANALOGS & DERIVATIVES/BLOOD/PHARMACOKINETICS
Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).